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electrophoresis




The diffusion of charged particles through fluids or gels (firm jelly-like substances) under the influence of an electric field. Particles with different sizes and charges diffuse at different rates, so that the effect can be used to separate and identify large molecules, such as proteins or fragments of DNA or RNA. The current forces the molecules through pores in a thin layer of gel, which is made so that its pores are just the right dimensions for separating molecules within a specific range of sizes and shapes. Smaller fragments usually travel further than large ones. The process is sometimes called gel electrophoresis. It is widely used to analyse body chemicals such as the various proteins in blood serum.


Related categories

   • ANALYTICAL CHEMISTRY
   • BIOCHEMISTRY

Source: National Human Genome Research Institute